Atp4b-IRES-CreERT2
品系全名
C57BL/6Smoc-Atp4bem1(IRES-CreERT2-WPRE-pA)Smoc
目录号
NM-KI-210118
品系状态
胚胎冻存
基因信息
基因名
Atp4b
品系描述
验证数据
Fig1. CreERT2-mediated recombination in the stomach of Atp4bCreERT2/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the stomach of Atp4bCreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. Some leakiness was detected prior to tamoxifen exposure.
Fig2. Detection of tdTomato (red) in various tissues of Atp4bCreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. Tdtomato was expressed in the glandular stomach. Tdtomato can also be detected in the pituitary, thymus, salivary glands, brown adipose tissue, spleen, pancreas, and muscle. Some leakiness was detected prior to tamoxifen exposure. TdTomato expression can not be detected in the brain, liver, lung, large intestine, small intestine, kidney or skin.
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Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。
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