hCB1

品系全名

C57BL/6Smoc-Cnr1em1(hCB1)Smoc

目录号

NM-HU-225027

品系状态

活体

导出PDF

基因信息

基因名
Cnr1

基因曾用名

CB1; CB-R; CB1R

NCBI ID

12801

MGI ID

104615

Ensembl ID

ENSMUSG00000044288

Pubmed

Cnr1

人类同源基因

CNR1

品系描述

利用同源重组，将小鼠Cnr1基因进行人源化修饰。

验证数据

image.png

Fig1. Detection of CB1 expression in cerebellum by RT-PCR. 

Wild type: only one band at 373 bp with primers F1/R1(mCb1);

Homozygous: only one band at 209 bp with primers F2/R2(hCB1);

Abbr. M, DNA marker; HO, homozygous;  WT, wild type.image.png

Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

image.png

Fig3. Detection of human CB1 expression in brain (Hippocampus) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

image.png

Fig4. Detection of human CB1 expression in CA1 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig5. Detection of human CB1 expression in CA3 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig6. Detection of human CB1 expression in dentate gyrus(DG) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig7. Detection of human CB1 expression in cerebellum in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.


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