hCD3E/hPD-L1

Fig1. Detection of CD3E expression in spleen and thymus by RT-PCR. Wild type: only one band at 465 bp with primers F1/R1(mCd3e);Homoygous: one band at 516 bp with primers F1/R1(recombinant hCD3E) and one band at 277 bp with primers F2/R2(hCD3E); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Detection of PD-L1 expression in spleen and thymus by RT-PCR. Wild type: only one band at 347 bp with primers F1/R1(mPd-l1);Homoygous: only one band at 329 bp with primers F2/R2(hPD-L1); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.