Muc2-2A-CreERT2-IRES-tdTomato

品系全名

C57BL/6Smoc-Muc2em1(2A-CreERT2-tdTomato-Wpre-pA)Smoc

目录号

NM-KI-200301

品系状态

精子冻存

导出PDF

基因信息

基因名

基因曾用名

MCM，wnn，2010015E03Rik

NCBI ID

17831

MGI ID

1339364

Ensembl ID

ENSMUSG00000025515

Pubmed

人类同源基因

MUC2

品系描述

将2A-CreERT2-tdTomato-Wpre-pA插入到小鼠Muc2基因终止密码子处。

验证数据

image.png

Fig 1. Active tdTomato expression was observed in the large intestine of Muc2CreERT2-IRES-tdTomato/+  mouse. WT, wild type as control.

image.png

Fig 2. Active tdTomato expression was observed  in the small intestine of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

image.png

Fig 3. No obvious tdTomato signal was observed in the stomach of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

image.png

Fig 4. Active tdTomato signal was observed in the colon of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

image.png

Fig 5. Active tdTomato signal was observed in the cecum of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

image.png

Fig 6. TdTomato signal was observed in the small intestine of Muc2CreERT2-IRES-tdTomato/+ mouse.WT, wild type as control.


image.png

Fig 7. No obvious tdTomato signal was observed in the stomach of Muc2CreERT2-IRES-tdTomato/+ mouse. WT, wild type as control.

参考文献

Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis
来源杂志：MDPI

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看