Lat-Y136F

品系全名

C57BL/6Smoc-Latem1(Y136F）Smoc

目录号

NM-KI-200249

品系状态

胚胎冻存

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基因信息

基因名
Lat

基因曾用名

pp36; p36-38

染色体位置

7

NCBI ID

16797

MGI ID

1342293

Ensembl ID

ENSMUSG00000030742

Pubmed

Lat

人类同源基因

LAT

品系描述

将Lat中第136位氨基酸由Y变为F，建立Lat点突变小鼠模型。
应用领域：罕见病模型--IgG4相关性疾病

疾病预测

IgG4相关性疾病
Igg4-Related Disease

近似模型的表型

MGI:2385971

参考文献

Aguado E, et al., Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science. 2002 Jun 14;296(5575):2036-40

验证数据

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Fig 1. Spleen (left) and mesenteric lymph nodes (right) from 8-week-old wild-type and Lat-Y136F mice.

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Fig 2. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in mesenteric lymph nodes (MLN) (n=3).

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Fig 3. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in peripheral blood (n=3).

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Fig 4. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in spleen (n=3).

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Fig 5. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in thymus (n=3).

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Fig 6. Serum IgG1 and IgE levels in homozygous Lat-Y136F mice (n=3-5).

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Fig 7. Histopathological analysis across organs in homozygous Lat-Y136F mice(n=3-4). Scale bar=100 μm; magnification,  200×.

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Fig 8. Serum IgG1 level and body weight post 3 weeks treatment in 4/7-week old homozygous Lat-Y136F mice (n=5).

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Fig 9. Wet weight and photos of spleen and mesenteric lymph nodes (MLN) upon prednisolone treatment in 7-week old homozygous Lat-Y136F mice (n=5).

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Fig. 10 Histopathology changes upon prednisolone treatment. Representative histopathological appearance of kidney and pancreas in 7-week-old prednisolone-treated and saline-treated control mice. Red arrows indicate inflammatory lesions and black arrows indicate fibrosis in the kidney and pancreas of C57 control mice, saline- or prednisolone-treated Lat-Y136F mice (n=5). Data are presented as mean and ± SEM. Scale bar=100 μm; magnification, 200×.

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