hIL17A/hIL17F

品系全名

C57BL/6Smoc-Il17atm1(hIL17A)Il17fem2(hIL17F)Smoc

目录号

NM-HU-200281

品系状态

活体

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基因信息

基因名
IL17A

基因曾用名

Il17; Ctla8; IL-17; Ctla-8; IL-17A

NCBI ID

16171

MGI ID

107364

Ensembl ID

ENSMUSG00000025929

Pubmed

IL17A

人类同源基因

IL17A

基因名
IL17F

基因曾用名

C87042; IL-17F

NCBI ID

257630

MGI ID

2676631

Ensembl ID

ENSMUSG00000041872

Pubmed

IL17F

人类同源基因

IL17F

品系描述

通过同源重组，将小鼠Il17a和Il17f基因进行人源化修饰。
应用领域：免疫相关；药物筛选

验证数据

NM-HU-hIL17A-hIL17F-1.png

Fig1. Analysis of IL17F gene expression in the kidney by RT-PCR. Mouse Il17f mRNA was detectable by P1/2(440bp), human IL17F mRNA was detectable by P3/4(259bp), M:100bp Plus DNA ladder from Transgen (BM311). The heterozygous KI mice express both human IL17F and mouse Il17f in the kidney.

NM-HU-hIL17A-hIL17F-2.png

Fig2. Analysis of IL17A gene expression in the spleen by RT-PCR in WT and IL17A/IL17F humanized mice . Mouse Il17a mRNA was detectable by P1/2(490bp), human IL17A mRNA was detectable by P3/4(443bp), M:100bp Plus DNA ladder from Transgen (BM311). The heterozygous KI mouse expresses both human IL17A and mouse Il17a in the spleen.

image.png

Fig. 3 Detection of hIL17A expression in serum, spleen and thymus homogenate by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type. 

Note. hIL17A/hIL17F and C57BL/6 mice were i.p. injected with LPS.

image.png

Fig. 4 Detection of hIL17F expression in serum, spleen and thymus homogenate by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type. 

Note. hIL17A/hIL17F and C57BL/6 mice were i.p. injected with LPS.

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