hIL6/hIL6R

Fig1. The expression of human IL6 in liver tissue of humanized IL6/IL6R mice was measured by qPCR. The results showed that the active expression of humanized IL6 can be detected in liver tissue of homozygous humanized IL6/IL6R mice after LPS Activation. 

Fig2. Expression of human IL6 in the serum of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6 can be detected in the serum collected from homozygous mice after LPS Activation. 

Fig3. Validation of human IL6 mRNA and protein expression by RT-PCR and ELISA in eyeball.

LPS was dissolved in endotoxin-free water and administered as a local eye drop at a concentration of 10 μg/μL (5 μL per eye). To ensure uniform distribution of the eye drop, the mouse was restrained for 1 minute. Four hours later, the whole eyeball was collected and homogenized to extract RNA and protein to detect hIL6 and hIL6R expression. (HO: humanized IL6 homozygous mice; WT:  wild type C57BL/6 mice)

Fig4. Detection of hIL-6R expression in eyeball by RT-PCR and ELISA(n=3). Humanized mice express human IL-6R. 

Abbr. HO, homozygous; WT, wild type.

Fig5. Detection of mIL-6R expression in liver by RT-PCR. Humanized mice do not express mouse IL-6R. 

Abbr. HO, homozygous; WT, wild type.

Fig6. Expression of human IL6R in the serum, liver and pancreas of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6R can be detected in the serum, liver and pancreas collected from homozygous mice. 

Fig7. Detection of hIL-6R expression in MM.1S cells.

Fig8. Detection of hIL-6R expression in T cells in hIL-6/hIL-6R dKI mice.

Fig9. Detection of hIL-6R expression in Myeloid cells, T cells and activated B cells in hIL-6/hIL-6R dKI mice. Note.  Splenocytes from C57BL/6 and homozygous IL-6/hIL-6R mice were stimulated with LPS in vitro. 

Fig10. Detection of hIL-6R expression in brainstem in hIL-6/hIL-6R dKI mice by IHC.

Fig11. Detection of hIL-6R expression in spleen in hIL-6 /hIL-6R dKI mice by IHC.

Fig12. Detection of hIL-6R expression in lung and thymus in hIL-6 /hIL-6R dKI mice by IHC.

Table 1 Blood routine test results of Hom hIL6/hIL6R mice (Data are presented as mean and ± SEM).

Table 2 Biochemistry examinations results of Hom hIL6/hIL6R mice (Data are presented as mean and ± SEM).