hIL21/hIL21R

Fig 1. Detection of IL21 expression in splenocyte by RT-PCR. 

Wild type: only one band at 272 bp with primers F1/R1 (mIl21);

Homozygous: only one band at 304 bp with primers F2/R2 (hIL21); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig 2. Detection of hIL21 expression in culture supernatants of splenocyte by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.

Fig 3. Detection of IL21R expression on spleen-derived activated T cells and B cells in hIL21/hIL21R dKI mice.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.