hIL2RA

Fig1. Detection of IL2RA expression by RT-PCR. 

Wild type: only one band at 203 bp with primers F2/R2(mIl2ra);

Homozygous: only one band at 251 bp with primers F1/R1(hIL2RA); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Development and validation of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) Targeting strategy for hIL2RA knokcinmice development; (B)hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice; (C)hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice. 

Fig3. Characterization of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse; (B)hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse. 

Fig4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Completed in collaboration with CrownBio)

(A)Tumor growth curves upon treatment; (B) Body weight change upon treatment.