hCFB

Fig1. Detection of CFB expression in liver by RT-PCR. 

Wild type: only one band at 441 bp with primers F1/R1(mCfb);

Homozygous: only one band at 442 bp with primers F2/R2(hCFB); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig2. Detection of human and mouse CFB expression in serum by ELISA (n=2). 

Abbr. HO, homozygous; WT, wild type.

Note: The mouse CFB ELISA kit cross-reacted with mCFB and humanized CFB.

Fig3. Detection of hCFB expression in serum by ELISA(n=3/group). 

Abbr. HO, homozygous; WT, wild type

Fig4. Detection of liver CFB mRNA remaining at Day 7 (A) and serum protein level (B) after single dose of test drug (n=3/group). (Data from a collaborator)

Fig5.Partial representative pictures of liver and kidney pathology in hCFB knockin mice. No obvious lesions were observed in hCFB knockin mice.