Ⅰ.In vitro Expression of MIA Paca-2-hCLDN18.2 cell line

Figure 1. hCLDN18.2 expression in MIA Paca-2 and MIA Paca-2-hCLDN18.2 cell lines

Ⅱ.Characterization of in vivo growth kinetics

To test the take rates, and the tumor growth characteristics of MIA Paca-2 -hCLDN18.2 cell line, cells were subcutaneously inoculated into the M-NSG mice.

Figure 2. Tumor growth curve of MIA Paca-2-hCLDN18.2 xenograft model model (n=5)

Figure 3. Body weight change of MIA Paca-2-hCLDN18.2 xenograft model model (n=5)

Ⅲ.Expression of hCLDN18.2 protein in tumors

To verify the hCLDN18.2 protein expression, the tumors were dissected and separated for single cell suspension.

Figure 4. The expression of hCLDN18.2 in humanized modified tumors by FACS