MC38-hPSMA (2)

Ⅰ.In vitro Expression of MC38-hPSMA cell line

The hPSMA protein expression in the wild-type MC38 and MC38- hPSMA cells were measured by Flow Cytometry.

Figure 1. hPSMA expression in wild-type MC38 and MC38-hPSMA cell line

Ⅱ. Characterization of in vivo growth kinetics

To test the take rates, and the tumor growth characteristics of MC38-hPSMA cell line, cells were subcutaneously inoculated into the C57BL/6 mice.

Figure 2. Tumor growth curve of MC38-hPSMA syngeneic model

Figure 3. Body weight change of MC38-hPSMA syngeneic model

Ⅲ. Expression of hPSMA protein in tumors

To verify the hPSMA protein expression, the tumors were dissected and separated for single cell suspension.

Figure 4. The expression of hPSMA in humanized modified tumors by FACS

Ⅳ.Efficacy verification

Murine colon cancer MC38-hPSMA cells were subcutaneously implanted into hCD28/hPSMA-C57BL/6 mice. Mice were grouped when tumor volume reached approximately 80-90 mm3, at which time they were treated with CD28×PSMA+mPD-1 antibody indicated in panel.

Figure 5. Antitumor activity of CD28×PSMA+mPD-1 antibody. MC38-hPSMA cells provided a powerful preclinical model for in vivo evaluation