hEPCAM

品系全名

C57BL/6Smoc-EpCAMtm1(hEpCAM)Smoc

目录号

NM-HU-210032

品系状态

精子冻存

导出PDF

基因信息

基因名
Epcam

品系描述

利用ES细胞打靶方式,将小鼠Epcam基因全部或者部分替换为人源EPCAM。从而表达人或人鼠嵌合EPCAM蛋白,取代小鼠内源Epcam蛋白的表达。
应用领域:免疫治疗;药物筛选

验证数据

1.png

Fig1. Analysis of mouse EPCAM and human EPCAM protein expression in the large intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

2.pngFig2. Analysis of mouse EPCAM and human EPCAM protein expression in the small intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

image.png

Fig3. Detection of human EpCAM expression in WT C57 and homozygous hEpCAM mice by IHC. Small intestine were collected from wild-type mice (+/+) and homozygous hEpCAM mice (HO/HO), and analyzed by IHC with anti-human EpCAM antibody. 


你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式,包括基因定点修饰的小鼠命名,比如:敲除、敲入、点突变等等,和随机转基因的小鼠命名。

查看