hPTPN1

Fig.1 Detection of PTPN1 expression in spleen by RT-PCR. 

Wild type: only one band at 214 bp with primers F1/R1(mPtpn1);

Homozygous: only one band at 206 bp with primers F2/R2(hPTPN1).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous.

Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.

Fig.3 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous; PC, positive control, Hela cells.

Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.