hINHBE

Fig.1 Detection of INHBE expression in liver by RT-PCR. 

Wild type: only one band at 192 bp with primers F1/R1 (mInhbe);

Homozygous: only one band at 217 bp with primers F2/R2 (hINHBE).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human INHBE expression in liver in hINHBE knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, homozygous; PC, positive control, Hep G2 cells.

Note. The anti-human INHBE Antibody cross-reacted with mouse INHBE and humanized INHBE. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.

Fig.3 Detection of hINHBE expression in serum by ELISA (n=3).

Note. The human INHBE Elisa kit cross-reacted with mouse INHBE and humanized INHBE.