Sox9-IRES-CreERT2

品系全名

C57BL/6Smoc-Sox9em(IRES-CreERT2-SV40pA)Smoc

目录号

NM-KI-204996

品系状态

活体

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基因信息

基因名
Sox9

品系描述

将IRES-CreERT2-SV40pA插入到小鼠Sox9基因终止密码子。
应用领域:Cre工具鼠,这些小鼠可用于产生他莫昔芬诱导的条件突变,用于研究干细胞分化过程中与 SOX9 表达相关的功能。

验证数据

image.png

图1 Sox9CreERT2/+;R26tdtomato/+ 小鼠心脏中可检测到tdTomato的表达。

经Tamoxifen诱导,Sox9CreERT2/+ ;R26tdtomato/+双转基因鼠,可在心脏中表达tdTomato。(见参考文献)

image.png

图 2 Sox9CreERT2/+; Rosa26tdTomato/+ 小鼠心脏中tdTomato的表达情况。

经Tamoxifen诱导后,Sox9CreERT2/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠心室和心房细胞中检测到tdTomato的表达。

image.png


图 3 Sox9CreERT2/+; Rosa26tdTomato/+ 小鼠毛囊中tdTomato的表达情况。

经Tamoxifen诱导后,Sox9CreERT2/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠毛囊和表皮细胞中检测到tdTomato的表达。

image.png

图 4 Sox9CreERT2/+; Rosa26tdTomato/+ 小鼠肝脏中tdTomato的表达情况。

经Tamoxifen诱导后,Sox9CreERT2/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠肝脏中检测到tdTomato的表达。

image.png

图 5 Sox9CreERT2/+; Rosa26tdTomato/+ 小鼠各组织中tdTomato的表达情况。TdTomato的表达可在心脏和肝脏的个别细胞,皮肤毛囊和表皮细胞,大脑、小脑,腺垂体、视网膜、胃、小肠和大肠的粘膜层、肺、颌下腺、肾、胰腺、脾脏、胸腺、卵巢的个别细胞中检测到。在棕色脂肪和肌肉中检测不到。(更多详细信息请联系我司技术顾问。)




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