HBV-Tg

将c1型HBV病毒1.0拷贝全长DNA转入小鼠，该转基因小鼠血清中HBsAg呈阳性，但无HBeAg、anti-HBs、anti-HBe或antiHBc表达，无HBV DNA复制。在该转基因小鼠模型的血清、肝脏和肾脏中可以检测到稳定表达的人乙肝病毒表面抗原，该小鼠模型为研究人类“乙型肝炎”的发病机制、治疗方法与药物筛选，提供了理想的动物模型和新型研究手段，具有重要的医药应用价值。

Fig1. Immuno-histochemical staining of HBsAg in the liver and kidney sections of #59 transgenic mice. Sections of liver (A) or kidney (B) tissues from #59 transgenic mice were stained for HBsAg.

Fig2.  Immuno-histochemical staining of HBcAg in the liver and kidney sections of #59 transgenic mice.

Sections of liver (A) or kidney (B) tissues from #59 transgenic mice were stained for HBcAg.

Tab1. Eight Selected Genes From the Kidney of #59 Mice Assayed by Microarray and Real-Time PCR

Fig3.  Dot blot immunoassay of complement C3 component in mouse serum and kidney tissue extract.

Reference

1. Ren J, Wang L, Chen Z, Ma ZM, Zhu HG, Yang DL, Li XY, Wang BI, Fei J, Wang ZG, Wen YM. Gene expression profile of transgenic mouse kidney reveals pathogenesis of hepatitis B virus associated nephropathy. J Med Virol. 2006 May;78(5):551-60. doi: 10.1002/jmv.20575. PMID: 16555286.

Expression of hepatitis B virus proteins in transgenic mice alters lipid metabolism and induces oxidative stress in the liver
来源杂志：JOURNAL OF HEPATOLOGY

An Altered Pattern of Liver Apolipoprotein A-I Isoforms Is Implicated in Male Chronic Hepatitis B Progression
来源杂志：ACS Publications

Hepatitis B virus (HBV) surface antigen interacts with and promotes cyclophilin a secretion: possible link to pathogenesis of HBV infection
来源杂志：Journal of Virology

Proteomic analysis of hepatitis B surface antigen positive transgenic mouse liver and decrease of cyclophilin A
来源杂志：Journal of Medical Virology

Transcriptional analysis of immune-related genes in dendritic cells from hepatitis B surface antigen (HBsAg)-positive transgenic mice and regulation of Fc gamma receptor IIB by HBsAg-anti-HBs complex
来源杂志：Journal of Medical Virology