hC5AR1/C5AR2

品系全名

C57BL/6Smoc-C5ar1tm1(hC5AR1)C5ar2tm1(hC5AR2)/Smoc

目录号

NM-HU-2000018

品系状态

胚胎冻存

导出PDF

基因信息

基因名
C5AR1

基因曾用名

C5aR; C5r1; Cd88; D7Msu1

NCBI ID

12273

MGI ID

88232

Ensembl ID

ENSMUSG00000049130

Pubmed

C5AR1

人类同源基因

C5AR1

基因名
C5AR2

基因曾用名

C5L2; Gpr77; E030029A11Rik

NCBI ID

319430

MGI ID

2442013

Ensembl ID

ENSMUSG00000074361

Pubmed

C5AR2

人类同源基因

C5AR2

品系描述

通过同源重组，将小鼠C5ar1和C5ar2基因进行人源化修饰。官网下架原因：专利申请。下架时间3-5个月。
应用领域：免疫治疗；药物筛选

验证数据

image.png

Fig1. Detection of C5AR1 and C5AR2 expression in spleen by RT-PCR. 

Wild type: only one band at 257 bp with primers F1/R1 (mC5ar1), only one band at 506 bp with primers F3/R3 (mC5ar2);

Homozygous: only one band at 313 bp with primers F2/R2 (hC5AR1),  only one band at 318 bp with primers F4/R4 (hC5AR2).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.image.png

Fig2. Detection of C5AR1 expression on myeloid cells in blood in hC5AR1 knockin mice.

image.png

Fig3. Detection of C5AR1 expression on myeloid cells in bone morrow in hC5AR1 knockin mice. 

image.png

Fig4. Detection of C5AR2 expression on myeloid cells in spleen in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

image.png

Fig5. Detection of C5AR2 expression on myeloid cells in bone marrow in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

image.png

Fig6. Detection of hC5AR2 expression on myeloid cells in peritoneal lavage fluids in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看