hLAG3

Fig1. Detection of LAG3 expression by RT-PCR. 

Wild type: only one band at 266 bp with primers 5/6(mLag3);

Heterozygous: one band at 266 bp with primers 5/6(mLAG3) and one band at 150 bp with primers 7/8(hLAG3); 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Detection of hLAG3 expression on T cells in hLAG3 KI mice.

Note. Splenocytes from C57BL/6 and homozygous hLAG3 mice were stimulated with anti-mCD3 antibody in vitro.

图3.  LAG3 人源化小鼠MC38 荷瘤模型体内抗肿瘤药效验证。

LAG3 人源化纯合子小鼠接种MC38 结肠癌细胞系，肿瘤生长至约50mm3 随机将动物分组(n=5)。结果显示：针对人的LAG3 抗体单独给药未见抗肿瘤效果，针对LAG3 抗体与PD1 抗体联合给药显示出显著的抗肿瘤效果。

图4. LAG3 人源化小鼠MC38 荷瘤模型体内验证Tencentriq 和针对LAG3 抗体联合给药抗肿瘤药效。

LAG3 人源化纯合子小鼠接种MC38 结肠癌细胞系，肿瘤生长至70–80 mm3 随机分组，3 天给药一次，连续给药6次。