Tert-CreERT2-2A-EGFP
品系全名
C57BL/6Smoc-Tertem1(CreERT2-2A-GFP-WPRE-PA)Smoc
目录号
NM-KI-18037
品系状态
胚胎冻存
基因信息
基因名
Tert
品系描述
在Tert基因起始密码子位点定点敲入CreERT2-2A-GFP-WPRE-PA表达框。
验证数据
Fig1. CreERT2 mediated recombination in small intestine of TertCreERT2-EGFP/+;Rosa26tdTomato/+ mouse.
Fig2. Detection of EGFP(green) and tdTomato(red) in various tissues of TertCreERT2-EGFP/+;Rosa26tdTomato/+ mouse. Expression tissues include: small intestine, lung, liver, salivary gland, kidney, pancreas. A few cells expressed in brain, stomach, colon, spleen, skin, thymus, ovary. Tissues that unexpressed include eye, heart, brown fat. No significant EGFP positive signal was detected. (6.5-months old, female) (For more detailed information please contact our technical advisor.)
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Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。
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