hIL12B

品系全名

C57BL/6Smoc-Il12betm3(hIL12B)/Smoc

目录号

NM-HU-2000057

品系状态

胚胎冻存

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基因信息

基因名
Il12b

品系描述

通过同源重组,将小鼠Il12b基因进行人源化修饰。
应用领域:细胞因子人源化模型;药物筛选

验证数据

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Fig1. Detection of IL12B expression in thymus by RT-PCR. 

Wild type: only one band at 260 bp with primers F1/R1(mIl12b);

Heterozygous: one band at 260 bp with primers F1/R1(mIl12b) and one band at 260 bp with primers F2/R2(hIL12B); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. Detection of hIL12B expression in plasma by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type. 

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

image.png

Fig 3. Detection of mIL12B expression in plasma by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type. 

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

image.png

Fig4. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.


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