hCSF1/hCSF1R(BALB/c)

Fig.1 Detection of CSF1 expression in spleen and lung by RT-PCR. 

Wild type: only one band at 299 bp with primers F1/R1(mCsf1);

Homozygous: only one band at 261 bp with primers F2/R2(hCSF1); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of CSF1R expression in spleen and lung by RT-PCR. 

Wild type: only one band at 233 bp with primers F1/R1(mCsf1r);

Homozygous: only one band at 345 bp with primers F2/R2(hCSF1R); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.3 Detection of hCSF1 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hCSF1/hCSF1R and BALB/c mice were i.p. injected with LPS for 6h.

Fig.4 Expression characterization of hCSF1R on Monocytes/ macrophages in Bone marrow in hCSF1/hCSF1R dKI mice.