Ⅰ.In vitro Expression of C1498-hCD24-Luc cell line

The human CD24 protein expression in the wild-type C1498 and C1498- hCD24-Luc cells were measured by Flow Cytometry.

Figure 1. hCD24 expression in wild-type C1498 and C1498-hCD24-Luc cell lines

Figure 2. Luciferase activity assay of C1498-hCD24-Luc cell line

Ⅱ. Characterization of in vivo growth kinetics

To verify whether there is the difference in tumor formation between the C1498-hCD24-Luc cell line and the wild-type C1498 cell line, both cells were subcutaneously inoculated into female C57BL/6 mice.

Figure 3. Tumor growth curve of C1498 and C1498-hCD24-Luc syngeneic model (n=5)

Figure 4. Body weight change of C1498 and C1498-hCD24-Luc syngeneic model (n=5)

Ⅲ.Expression of hCD24 protein in tumors

Figure 5. The expression of hCD24 in humanized modified tumors by FACS