Cd8a-2A-tdTomato-2A-Luc

品系全名

C57BL/6Smoc-Cd8aem(T2A-tdTomato-P2A-Luciferase)1Smoc

目录号

NM-KI-225025

品系状态

精子冻存

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基因信息

基因名
Cd8a

品系描述

将T2A-tdTomato-P2A-Luciferase插入到小鼠Cd8a基因终止密码子处。
应用领域:荧光示踪小鼠

验证数据

Fig.1.png

Fig.1 IVIS automatic exposure.According to the imaging process, the mice were injected with D-Luciferin, and the abdomen, back, and flanks were imaged in vivo. 

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Fig.2 IVIS automatic exposure: ex vivo. According to the imaging process, D-Luciferin was injected, and the mouse brain, heart, lung, kidney, liver,  spleen, thymus, inguinal lymph node, axial lymph node (Axial LN), muscle, and the entire digestive system  (stomach to colon) were taken for imaging.

Fig.3.pngFig.3 Detection of tdTomato in mice thymus by FACS.

Fig.4.png

Fig.4 Detection of tdTomato in mice axillary lymph by FACS.

Fig.5.png

Fig.5 Detection of tdTomato in mice inguinal lymph by FACS.

Fig.6.pngFig.6 Detection of tdTomato in mice spleen by FACS.

Fig.7.pngFig.7 Detection of tdTomato in mice PB by FACS. 

*The validation data above were completed in cooperation with Crownbio.


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