hPSGL-1(2)

品系全名

C57BL/6Smoc-Selplgem2(hSELPLG)Smoc

目录号

NM-HU-210026

品系状态

胚胎冻存

导出PDF

基因信息

基因名
Selplg

基因曾用名

CD162， Psgl1， Selp1， Selpl， Psgl-1

NCBI ID

20345

MGI ID

106689

Ensembl ID

ENSMUSG00000048163

Pubmed

Selplg

人类同源基因

SELPLG

品系描述

利用CRISPR基因编辑技术，将小鼠Selplg基因全部或者部分替换为人源SELPLG。从而表达人或人鼠嵌合SELPLG蛋白，取代小鼠内源Selplg蛋白的表达。
应用领域：免疫治疗；药物筛选

验证数据

image.png

Fig1. Detection of PSGL-1 expression in thymus and spleen by RT-PCR. Wild type: only one band at 248 bp with primers F1/R1(mPsgl-1);Homozygous: only one band at 237 bp with primers F2/R2(hPSGL-1); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of hPSGL-1 expression on B cells in blood in hPSGL-1 knockin mice.

image.png

Fig3. Detection of hPSGL-1 expression on Monocytes and Granulocytes in blood in hPSGL-1 knockin mice.

image.png

Fig4. Detection of hPSGL-1 expression on B cells in bone morrow in hPSGL-1 knockin mice.

image.png

Fig5. Detection of hPSGL-1 expression on Monocytes and Granulocytes in bone morrow in hPSGL-1 knockin mice.

image.png

Fig6. Detection of hPSGL-1 expression on T cells in bone morrow in hPSGL-1 knockin mice.

image.png

Fig7. Detection of hPSGL-1 expression on NKs and DCs in bone morrow in hPSGL-1 KI mice.

image.png

Fig8. Detection of hPSGL-1 expression on B cells in spleen in hPSGL-1 KI mice.

image.png

Fig9. Detection of hPSGL-1 expression on Monocytes and Granulocytes in spleen in hPSGL-1 knockin mice.

image.png

Fig10. Detection of hPSGL-1 expression on T cells in Thymus in hPSGL-1 knockin mice.

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看