Foxp3-IRES-DTR-2A-tdTomato(BALB/c)

品系全名

BALB/cAnSmoc-Foxp3em9(IRES-DTR-2A-tdTomato)Smoc

目录号

NM-KI-200213

品系状态

胚胎冻存

导出PDF

基因信息

基因名
Foxp3

基因曾用名

sf; JM2; scurfin

NCBI ID

20371

MGI ID

1891436

Ensembl ID

ENSMUSG00000039521

Pubmed

Foxp3

人类同源基因

FOXP3

品系描述

将IRES-DTR-2A-tdTomato共表达结构插入到小鼠Foxp3基因终止密码子处。
应用领域：DTR工具鼠；调节性T细胞

验证数据

CT 26 tumor cells were injected into FOXP3-DTR mice administered with different treatment, and Balb/c mice, respectively. And tumor growth curves of each group were recorded.

image.png

Fig 1. The growth of CT26 tumors in Foxp3-IRES-DTR-2A-tdTomato mice administered with different treatment and Balb/c mice. (In collaboration with CrownBio)


Proportion of different type-specific T cells and macrophages in peripheral blood of each group analyzed using FACS

image.png

Fig 2. The percentage of different type-specific T cells and macrophages in peripheral blood of CT26 tumor-bearing Foxp3-IRES-DTR-2A-tdTomato mice administered with different treatment and Balb/c mice is detected by FACS. (In collaboration with CrownBio)


Proportion of different type-specific T cells and macrophages in spleen and tumor of each group analyzed using FACS

image.png

Fig 3. The percentage of different type-specific T cells and macrophages in spleen and tumor of of CT26 tumor-bearing Foxp3-IRES-DTR-2A-tdTomato mice administered with different treatment and Balb/c mice is detected by FACS. (In collaboration with CrownBio)

image.png

Fig 4. DTR  mediated cell knockout  in spleen of Foxp3-DTR/tdTomato mice (BALB/c).

image.png

Fig 5. DTR  mediated cell knockout  in mesenteric lymph nodes of Foxp3-DTR/tdTomato mice(BALB/c). 

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看